A sensitive noninvasive method for monitoring successful liver-directed gene transfer of the low-density lipoprotein receptor in Watanabe hyperlipidemic rabbits in vivo.
Identifieur interne : 003142 ( Main/Exploration ); précédent : 003141; suivant : 003143A sensitive noninvasive method for monitoring successful liver-directed gene transfer of the low-density lipoprotein receptor in Watanabe hyperlipidemic rabbits in vivo.
Auteurs : RBID : pubmed:14724675English descriptors
- KwdEn :
- Adenoviridae (genetics), Animals, Female, Gene Expression, Genetic Therapy (methods), Genetic Vectors (administration & dosage), Hyperlipoproteinemias (metabolism), Hyperlipoproteinemias (therapy), Indium Radioisotopes (diagnostic use), Injections, Intravenous, Lipoproteins, LDL (administration & dosage), Lipoproteins, LDL (pharmacokinetics), Liver (metabolism), Liver (radionuclide imaging), Rabbits, Receptors, LDL (genetics), Receptors, LDL (metabolism), Transduction, Genetic (methods), Transgenes, Treatment Outcome.
- MESH :
- chemical , administration & dosage : Lipoproteins, LDL.
- chemical , diagnostic use : Indium Radioisotopes.
- administration & dosage : Genetic Vectors.
- genetics : Adenoviridae, Receptors, LDL.
- metabolism : Hyperlipoproteinemias, Liver, Receptors, LDL.
- methods : Genetic Therapy, Transduction, Genetic.
- chemical , pharmacokinetics : Lipoproteins, LDL.
- radionuclide imaging : Liver.
- therapy : Hyperlipoproteinemias.
- Animals, Female, Gene Expression, Injections, Intravenous, Rabbits, Transgenes, Treatment Outcome.
Abstract
Noninvasive tools to quantitate transgene expression directly are a prerequisite for clinical gene therapy. We established a method to determine location, magnitude, and duration of low-density lipoprotein (LDL) receptor (LDLR) transgene expression after adenoviral gene transfer into LDLR-deficient Watanabe hypercholesterolemic rabbits by following tissue uptake of intravenously injected (111)In-labeled LDL using a scintillation camera. Liver-specific tracer uptake was calculated by normalizing the counts measured over the liver to counts measured over the heart that represent the circulating blood pool of the tracer (liver/heart (L/H) ratio). Our results indicate that the optimal time point for transgene imaging is 4 h after the tracer injection. Compared with control virus-injected rabbits, animals treated with the LDLR-expressing adenovirus showed seven-fold higher L/H ratios on day 6 after gene transfer, and had still 4.5-fold higher L/H ratios on day 30. This imaging method might be a useful strategy to obtain reliable data on functional transgene expression in clinical gene therapy trials of familial hypercholesterolemia.
DOI: 10.1038/sj.gt.3302206
PubMed: 14724675
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A sensitive noninvasive method for monitoring successful liver-directed gene transfer of the low-density lipoprotein receptor in Watanabe hyperlipidemic rabbits in vivo.</title><author><name sortKey="Tietge, U J F" uniqKey="Tietge U">U J F Tietge</name><affiliation wicri:level="3"><nlm:affiliation>Department of Medicine and NWFZ, Charité Campus Mitte, Humboldt University, Berlin, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Medicine and NWFZ, Charité Campus Mitte, Humboldt University, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Cichon, G" uniqKey="Cichon G">G Cichon</name></author><author><name sortKey="B Ttner, C" uniqKey="B Ttner C">C Büttner</name></author><author><name sortKey="Genschel, J" uniqKey="Genschel J">J Genschel</name></author><author><name sortKey="Heeren, J" uniqKey="Heeren J">J Heeren</name></author><author><name sortKey="Gielow, P" uniqKey="Gielow P">P Gielow</name></author><author><name sortKey="Grewe, N" uniqKey="Grewe N">N Grewe</name></author><author><name sortKey="Dogar, M" uniqKey="Dogar M">M Dogar</name></author><author><name sortKey="Beisiegel, U" uniqKey="Beisiegel U">U Beisiegel</name></author><author><name sortKey="Manns, M P" uniqKey="Manns M">M P Manns</name></author><author><name sortKey="Lochs, H" uniqKey="Lochs H">H Lochs</name></author><author><name sortKey="Burchert, W" uniqKey="Burchert W">W Burchert</name></author><author><name sortKey="Schmidt, H H J" uniqKey="Schmidt H">H H-J Schmidt</name></author></titleStmt><publicationStmt><date when="2004">2004</date><idno type="RBID">pubmed:14724675</idno><idno type="pmid">14724675</idno><idno type="doi">10.1038/sj.gt.3302206</idno><idno type="wicri:Area/Main/Corpus">003308</idno><idno type="wicri:Area/Main/Curation">003308</idno><idno type="wicri:Area/Main/Exploration">003142</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenoviridae (genetics)</term><term>Animals</term><term>Female</term><term>Gene Expression</term><term>Genetic Therapy (methods)</term><term>Genetic Vectors (administration & dosage)</term><term>Hyperlipoproteinemias (metabolism)</term><term>Hyperlipoproteinemias (therapy)</term><term>Indium Radioisotopes (diagnostic use)</term><term>Injections, Intravenous</term><term>Lipoproteins, LDL (administration & dosage)</term><term>Lipoproteins, LDL (pharmacokinetics)</term><term>Liver (metabolism)</term><term>Liver (radionuclide imaging)</term><term>Rabbits</term><term>Receptors, LDL (genetics)</term><term>Receptors, LDL (metabolism)</term><term>Transduction, Genetic (methods)</term><term>Transgenes</term><term>Treatment Outcome</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Lipoproteins, LDL</term></keywords><keywords scheme="MESH" type="chemical" qualifier="diagnostic use" xml:lang="en"><term>Indium Radioisotopes</term></keywords><keywords scheme="MESH" qualifier="administration & dosage" xml:lang="en"><term>Genetic Vectors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Adenoviridae</term><term>Receptors, LDL</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Hyperlipoproteinemias</term><term>Liver</term><term>Receptors, LDL</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genetic Therapy</term><term>Transduction, Genetic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Lipoproteins, LDL</term></keywords><keywords scheme="MESH" qualifier="radionuclide imaging" xml:lang="en"><term>Liver</term></keywords><keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Hyperlipoproteinemias</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Gene Expression</term><term>Injections, Intravenous</term><term>Rabbits</term><term>Transgenes</term><term>Treatment Outcome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Noninvasive tools to quantitate transgene expression directly are a prerequisite for clinical gene therapy. We established a method to determine location, magnitude, and duration of low-density lipoprotein (LDL) receptor (LDLR) transgene expression after adenoviral gene transfer into LDLR-deficient Watanabe hypercholesterolemic rabbits by following tissue uptake of intravenously injected (111)In-labeled LDL using a scintillation camera. Liver-specific tracer uptake was calculated by normalizing the counts measured over the liver to counts measured over the heart that represent the circulating blood pool of the tracer (liver/heart (L/H) ratio). Our results indicate that the optimal time point for transgene imaging is 4 h after the tracer injection. Compared with control virus-injected rabbits, animals treated with the LDLR-expressing adenovirus showed seven-fold higher L/H ratios on day 6 after gene transfer, and had still 4.5-fold higher L/H ratios on day 30. This imaging method might be a useful strategy to obtain reliable data on functional transgene expression in clinical gene therapy trials of familial hypercholesterolemia.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">14724675</PMID><DateCreated><Year>2004</Year><Month>03</Month><Day>18</Day></DateCreated><DateCompleted><Year>2004</Year><Month>07</Month><Day>21</Day></DateCompleted><DateRevised><Year>2012</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0969-7128</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><Issue>7</Issue><PubDate><Year>2004</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Gene therapy</Title><ISOAbbreviation>Gene Ther.</ISOAbbreviation></Journal><ArticleTitle>A sensitive noninvasive method for monitoring successful liver-directed gene transfer of the low-density lipoprotein receptor in Watanabe hyperlipidemic rabbits in vivo.</ArticleTitle><Pagination><MedlinePgn>574-80</MedlinePgn></Pagination><Abstract><AbstractText>Noninvasive tools to quantitate transgene expression directly are a prerequisite for clinical gene therapy. We established a method to determine location, magnitude, and duration of low-density lipoprotein (LDL) receptor (LDLR) transgene expression after adenoviral gene transfer into LDLR-deficient Watanabe hypercholesterolemic rabbits by following tissue uptake of intravenously injected (111)In-labeled LDL using a scintillation camera. Liver-specific tracer uptake was calculated by normalizing the counts measured over the liver to counts measured over the heart that represent the circulating blood pool of the tracer (liver/heart (L/H) ratio). Our results indicate that the optimal time point for transgene imaging is 4 h after the tracer injection. Compared with control virus-injected rabbits, animals treated with the LDLR-expressing adenovirus showed seven-fold higher L/H ratios on day 6 after gene transfer, and had still 4.5-fold higher L/H ratios on day 30. This imaging method might be a useful strategy to obtain reliable data on functional transgene expression in clinical gene therapy trials of familial hypercholesterolemia.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tietge</LastName><ForeName>U J F</ForeName><Initials>UJ</Initials><Affiliation>Department of Medicine and NWFZ, Charité Campus Mitte, Humboldt University, Berlin, Germany.</Affiliation></Author><Author ValidYN="Y"><LastName>Cichon</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Büttner</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Genschel</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Heeren</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Gielow</LastName><ForeName>P</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Grewe</LastName><ForeName>N</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Dogar</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Beisiegel</LastName><ForeName>U</ForeName><Initials>U</Initials></Author><Author ValidYN="Y"><LastName>Manns</LastName><ForeName>M P</ForeName><Initials>MP</Initials></Author><Author ValidYN="Y"><LastName>Lochs</LastName><ForeName>H</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Burchert</LastName><ForeName>W</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Schmidt</LastName><ForeName>H H-J</ForeName><Initials>HH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Gene Ther</MedlineTA><NlmUniqueID>9421525</NlmUniqueID><ISSNLinking>0969-7128</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Indium Radioisotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Lipoproteins, LDL</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance>Receptors, LDL</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N">Adenoviridae</DescriptorName><QualifierName MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Genetic Therapy</DescriptorName><QualifierName MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Genetic Vectors</DescriptorName><QualifierName MajorTopicYN="N">administration & dosage</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Hyperlipoproteinemias</DescriptorName><QualifierName MajorTopicYN="N">metabolism</QualifierName><QualifierName MajorTopicYN="Y">therapy</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Indium Radioisotopes</DescriptorName><QualifierName MajorTopicYN="Y">diagnostic use</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Injections, Intravenous</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Lipoproteins, LDL</DescriptorName><QualifierName MajorTopicYN="Y">administration & dosage</QualifierName><QualifierName MajorTopicYN="N">pharmacokinetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Liver</DescriptorName><QualifierName MajorTopicYN="Y">metabolism</QualifierName><QualifierName MajorTopicYN="N">radionuclide imaging</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Rabbits</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Receptors, LDL</DescriptorName><QualifierName MajorTopicYN="Y">genetics</QualifierName><QualifierName MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Transduction, Genetic</DescriptorName><QualifierName MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Transgenes</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N">Treatment Outcome</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>1</Month><Day>16</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>7</Month><Day>22</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>1</Month><Day>16</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">14724675</ArticleId><ArticleId IdType="doi">10.1038/sj.gt.3302206</ArticleId><ArticleId IdType="pii">3302206</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=IndiumV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003142 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003142 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= *** parameter Area/wikiCode missing ***
|area= IndiumV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:14724675
|texte= A sensitive noninvasive method for monitoring successful liver-directed gene transfer of the low-density lipoprotein receptor in Watanabe hyperlipidemic rabbits in vivo.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:14724675" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a IndiumV2
| This area was generated with Dilib version V0.5.76. |  |